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Peripheral blood monocytes were prepared from human blood as previously described [66] and were differentiated to macrophages as described [33].
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Sinularia soft corals were differentiated into morphospecies based on growth form; all other anthozoans and sponges were differentiated to species.
The Mb samples were derived from biopsies, and aliquots were differentiated to Mt. Importantly, all had been characterized immunocytochemically as previously described [ 11].
We then examined whether cells that were abnormally positioned in the IZ and VZ/SVZ were differentiated to neurons or not.
After 24 h, non-adherent cells were removed and adherent monocytes were differentiated to macrophages for 7 10 days, with fresh medium changes every second day.
C2C12 and L6-GLUT4myc myoblasts were differentiated to myotubes as described previously [ 21].
Human ES and IMP cells were differentiated to the cardiac lineage as previously described (Lian et al., 2012).
For each vaccination, part of the PBMC was thawed, and adherent cells were differentiated to immature DC as described (Thurner et al, 1999).
Short interfering RNAs targeting TRIM23 (siTRIM23) and a non-targeting control siRNA (siControl) were introduced into human primary visceral preadipocytes, and the preadipocytes were differentiated to mature adipocytes.
To confirm reproducibility of the differentiation process, BHX HiPSCs along with H9 HESCs were differentiated to LM and subsequently to an epicardial lineage with WNT3A+BMP4+RA.
PBMCs, SFMCs and T cell-depleted SFMCs were differentiated to osteoclasts as described previously [ 9, 22, 30].
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