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After 30-min incubation, the samples were filtered through a nitrocellulose membrane and washed with a reaction buffer.
Polymers with unsaturated pendant groups were spin-coated on a photoinitiator tethered surface, which was then photoirradiated and washed with a solvent.
The carcass is sawed in half and washed with a lactic-acid solution before it's moved to a chilling compartment.
The beads were collected by centrifugation and washed with a lysis buffer 5 times.
The as-synthesized nanocomposites were centrifugated and washed with a large amount of distilled water and then with methanol.
The aqueous phase was extracted with CH2Cl2 (3×) and washed with a saturated aqueous solution of NaCl (60 ml volume).
Then, the product mixture was filtered and washed with a large amount of deionized water followed by drying at 100 °C in an oven for 24 h.
The synthesis process was started when the catalyst layer, externally heated and washed with a stream of nitrogen, achieved 310°C.
Mycelia were harvested and washed with a large amount of distilled water and then dried at 60 °C to a constant biomass.
Then, the products were filtered off and washed with a small amount of distilled water before being dried in ambient air.
The precipitate was isolated and washed with a plenty of water and dried to a constant weight in vacuo for 4 days at a temperature of 45 °C.
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