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Total RNA of mouse colon was extracted using ISOGEN Nippon Gene Co..) and was quantified by NanoDrop (NanoDrop 2000, Thermo Scientific).
Thereby, abrasion of adsorbed proteins by contact sliding was identified as prevailing protein degradation mechanism and was quantified by an increase in turbidity and by monomer loss.
Matrix inhomogeneity was controlled by changing the casein concentration, and was quantified by confocal laser scanning microscopy (CLSM) and image analysis.
The thickness of the oxidized region at long oxidation times is termed the critical thickness Lc and was quantified by various analyses.
Each section was scanned using a high-resolution scanner (Epson, GT-X820, Nagano, Japan) and was quantified by ImageJ (NIH, Bethesda, MD, USA).
Time engaged in floating behavior, which included small movements necessary for the rat to keep its head above water, during a 5 m test session served as the dependent measure and was quantified by EthoVision video tracking software.
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The phosphorylated bands were detected from the autoradiogram and were quantified by densitometry using QuantityOne software.
Protein eluates were analysed by SDS-PAGE and were quantified by A280 measurement.
Mitochondria were selected based on a threshold and were quantified by using Metamorph (Molecular Devices Corporation).
The latter mimics cell micro-motility and is quantified by the cell diffusion constant.
Peptides were purified by liquid chromatography/mass spectrometry to >95% purity and were quantified by amino-acid analysis.
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