Close Persons Questionnaire items enquired about relationships with the husband, mother and mother-in-law and depression was ascertained using the Edinburgh Postnatal Depression Scale at the each assessments.
We focus on a group of miRNAs, whose increased expression in normal breast and PMC42 was ascertained using three independent methods and whose clustering is not algorithm-dependent.
As explained in the section describing "Motion classification", the SVM classification performance depends on selection of kernel parameter (upgamma) and penalty parameter C. The optimal combination of (upgamma) and C was ascertained using a grid search within the training data.
RNA quantity and quality was ascertained using a NanoDrop™ 1000 spectrophotometer (Thermo Fisher Scientific Ltd., Waltham, MA, USA) and an Agilent 2100 Bioanalyzer with the RNA 6000 Nano LabChip kit (Agilent Technologies Ltd., Cork, Ireland).
Total RNA was quantified using spectrophotometry based on absorbance at 260 nm, and integrity was ascertained using the Agilent 2100 Bioanalyzer (Agilent Technologies, Kista, Sweden).
Incident diabetes diagnosed between 1996 and 2010 was ascertained using the Ontario Diabetes Database, a validated registry of persons diagnosed with diabetes (sensitivity = 86%, specificity = 97%).
Acute myocardial infarction was diagnosed in 17% of cases and accuracy was ascertained using AUC (area under the receiver operating characteristic curve).
From 1992 to 2001, production of Stx1 and Stx2 was ascertained using the GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) with some minor modifications [ 28].
21 In both phases, CMD (depression, anxiety and somatoform disorder) was measured using the Primary Care Evaluation of Mental Disorders Patient Health Questionnaire (PRIME MD PHQ) and PTSD was ascertained using Section-K of the Composite International Diagnostic Interview (CIDI-K).
Hence, whether xCT mRNA levels was altered following aglycemia in pure astrocyte cultures or in mixed cortical cultures containing both astrocytes and neurons was ascertained using quantitative PCR analysis.
