Membranes were then washed and visualized using a Licor Odyssey Infrared imaging system.
Stained cultures on coverslips were mounted on glass slides and visualized using a confocal laser scanning microscope (Zeiss, Oberkochen, Germany).
Finally, sections were counterstained with 4′,6-diamidino-2-phenylindole (H-1200, Vector Laboratories), and visualized using a fluorescence microscope.
Immune complexes were incubated with corresponding secondary antibodies and visualized using a chemiluminescent peroxidase substrate (Roche, Mannheim, Germany; or Thermo Scientific, Rockford, USA).
Cells were finally mounted in prolong anti-fade with DAPI (Invitrogen) and visualized using a confocal microscope SP2 (Leica, Wetzlar, Germany).
The samples were then placed in glass bottom 35 mm-dishes (MatTek Corporation, P35G-1.5 14-C P35G-1.5 14-C P35G-1.5 14-Cnd visualized using a Nikon A1R confilledlaser-scanning microscope equipped with GaAsP detectors.
DNA was transferred to MSI nylon membranes (MSI, MA, USA) and visualized using a Phototope-Star Detection Kit (New England Biolabs, MA, USA).
However, the effectiveness of CCTV coverage is often achieved through design experience and trial-and-error, instead of being evaluated and visualized using a robust approach.
Samples were run on 20% denaturing polyacrylamide gels and visualized using a PhosphorImager.
Fixed worms were mounted on slides and visualized using a Zeiss Axioscope 2+ microscope.
Samples were run on 4.5% polyacrylamide (19∶1) gel and visualized using a PhosphorImager.
