The repression of the Pttg1 locus at the transcriptional level was also confirmed at the level of protein expression by means of WB assays which also confirmed and validated the patterns of microarray-based differential expression identified in pancreatic islets for several other randomly selected, repressed or induced loci listed in Table S1 (Additional file 1).
Finally, we give an example of a RT pattern that illustrates these UML extensions, where we implement the proposed profile and we validate the pattern diagrams using the constraints we have proposed.
To examine and potentially validate the pattern of COX5A upregulation in humans reported in Uddin et al. [ 24], a SYBR green-based quantitative real-time quantitative PCR (qRT-PCR) strategy was designed to test the same individuals and species originally investigated by the microarray experiments: Homo sapiens (N = 3), Pan troglodytes (N = 1), Gorilla gorilla (N = 1) and Macaca mulatta (N = 3).
A second set of reference compounds known to also have that toxicological MoA were used to test and validate the discriminating pattern.
The main objective of this study is to counterpart numerical model and experimental studies for rotating flows associated with dough kneading, and validate the flow patterns generated.
This study uses Computational Fluid Dynamics (CFD) to predict, analyze and validate the deposition patterns in a human lung for a Budesonide drug delivered from the Novolizer Dry Powder Inhaler device.
Molecular biologists who study any one of these pathways may select candidate gene targets of these pathways uncovered by this study and validate the expression patterns both in experimental models (models using prostate cells in particular) and in human prostate tumor specimens.
The objectives of this study were to apply the cDNA-AFLP technique to the wheat-stripe rust pathogen interaction pathosystem, identify genes regulated during the compatible interaction between the host and pathogen, and validate the expression patterns for some of the regulated genes through quantitative real-time polymerase chain reaction (qRT-PCR).
Thus the immunohistochemical and real-time PCR profiling validate the pattern defined by the inverse reporter, indicating that miR-132 expression increases as granule neurons differentiate and mature.
The frequency represents the number of association rules that validate the pattern with confidence and support values of the rule.
