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Caliban's capacity for a sort of nobility is knitted into his music and underlined in the plot.
As commented by Geels, and underlined in the current article, 'this asymmetry runs counter to the initial MLP-formulations and the emphasis on multi-level alignments' ([16], p. 23).
This position is summed up and underlined in the statement, now consolidated in deontological standards, that 'the physician must not undertake diagnostic and/or therapeutic activity without the acquisition of the patient's explicit and informed consent'.
Many of these could not be assigned because they were within 10 Å of the heme iron with its five unpaired electrons (bolded and underlined in the list above); however, it was possible to assign several of the residues with backbone N values of <10 Å [L57 (9.49 Å), Y105 (9.75 Å), L123 (7.75 Å), and Y134 (9.59 Å)].
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Therefore hrpA2 should have been shown in boldface and underlined in Figure 1 of the manuscript [ 1].
The insert was excised with BamHI and NotI (underlined in the primers) and ligated into corresponding sites of the pET-30a vector (Novagen, Madison, WI, USA) to produce plasmid pETPG1.
As expected, the genes coding for classical UPR targets are induced both in Hac1 overproducing and in DTT stressed cells, and genes underlined in the following paragraphs have been identified as UPR targets in previous studies.
Variants of the native peptide sequence (NN LR L S FGKR) were produced with a single Met substituted at each of the three positions (P−3, −1 and +1; underlined in the sequence) thought to be important for hydrophobic interactions with the protein kinase.
These products were subsequently directionally cloned into pRG970b (Van den Eede et al. 1992) using XmaI and BamHI (underlined in the primer sequence), such that the traA promoter was transcriptionally fused to lacZ.
Restriction sites for BamHI and EcoRI are underlined in the forward and reverse primers, respectively.
We directionally cloned the PCR product into pBBR1MCS-derived vector pZLQ (Luo and Farrand 1999) using the NdeI and HindIII sites underlined in the primer sequences.
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