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Single colonies were selected and transformed with a third, HIS3-containing plasmid encoding Sir2 or GAPDH.
In the second approach, the wild type strain CC-124 was employed as background strain and transformed with a paromomycine resistance cassette derived from plasmid pSL18 [ 21].
The products of RT-PCR were purified and cloned into the pGEM-T Easy vector (Promega, USA) and transformed with a JM109 competent cell.
The coding sequences of two proteins derived from hyperthermophilic microorganisms have been synthesized and transformed with a specially prepared expression vector into a bacterial expression system without DNA digestion or ligation.
Lastly, a segment of length w/2 was randomly chosen from the smooth vector and transformed with a logarithmic function in order to match the SF sensitivity of the human visual system.
Successfully matched spots were compared across groups according to SA (standardized abundance), where a sample spot fluorescence volume (Cy5) is divided by the standard spot volume (Cy3), normalized with ratiometric normalization and transformed with a log10 function.
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Following selection with puromycin, a Δ TbGT3:: PAC mutant was selected and transformed with an ectopic, tetracycline-inducible, copy of TbGT3, introduced into the ribosomal DNA locus under phleomycin selection (Wirtz et al. 1999).
Buffy coat was prepared, lymphocytes were pelleted, and transformed with Epstein Barr virus and cyclosporin A following standard procedures.
The plantlets were cut at the soil line and transformed with A. rhizogenes grown to an OD600 of 0.5.
Ms. Antoni's photographs of her parents dressed as each other and transformed with makeup, wigs and prosthetics offer a sly, grown-up commentary on the gender identifications and confusions children experience via their parents.
Compound mutant strains were generated from strains bearing single-copy mutant H3 (K9A or K14A) and clr4Δ, and were transformed with a plasmid expressing 3xFlag-clr4 + under control of its native regulatory sequences.
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