Sentence examples for and transform with the from inspiring English sources

Supporting one very delicate bass solo of Okegwo during the program, he took the butt end of a stick and tapped in the sweet spot of a cymbal, just enough to have the sound mix, radiate, elevate and transform, with the audience reacting in a very pleasant "Ooooh".

Nourseothricin-resistant, Geneticin-sensitive transformants were selected and transformed with the PUF4 :: KanMX amplicon, as described previously (see Supporting Information, Table S4 for details).

These transformants were re-grown and transformed with the third plasmid encoding the protein to be studied.

Yeast strains with deletions in genes encoding Pho85-associated cyclins were taken from the yeast deletion collection and transformed with the multi-copy ADH1 promoter-ADR1 plasmid.

Each PCR fragment was TA cloned into pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA) and transformed with the TOP10-competent cells (Invitrogen, Carlsbad, CA).

The cells were made electrocompetent and transformed with the integration vector pP21KF3T5b-crtEIBipi.

Cells were made chemically competent and transformed with the pET15b plasmid.

Arabidopsis mesophyll cell protoplasts were obtained and transformed with the GFP fusion construct according to Wu et al. (2009).

The resulting strain was recovered on a YPD selective plate lacking uracil and transformed with the YIplac128 plasmid, linearized with EcoRV within the LEU2 gene.

Values represent the mean ± standard deviation of three to six individual plants homozygous for the respective T-DNA insertion and transformed with the respective expression constructs.

Competent E. coli LE392 cells were prepared by the calcium chloride procedure [ 28] and transformed with the above described expression plasmids.