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At the start of each trial, an individual was moved in its isolation chamber to the testing area and transferred with a net to the assay tank for testing.
From these raw stock cultures, a small volume was poured into Petri dishes; individual females were selected and transferred with a pipette to 12-well tissue plates (one female per well).
The condensate was filled in a headspace vial and transferred with a gas-tight syringe to the autosampler vials which contained the tester strains and the S9-mix or equivalent amounts of phosphate buffered saline.
For high throughput analyses of the YKO knockout collection, a 96-well plate was pinned onto YPD agar plates from −80 °C stocks (without thawing), incubated at 30 °C over two nights and transferred with a pin tool into 200 μl YPD for 48 h at 30 °C.
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Subsequent passages were identical with blood collected and transferred with an average of 5 ± 1 day interval.
The heads of mosquitoes were individually removed with a scalpel and transferred with forceps into a 1.5 mL Diethylpyrocarbonate (DEPC) treated microcentrifuge tube labeled "24.1" and 100 μL of RNA later® were added.
After 15 minutes positive, negative and all allergen reactions were drawn up on the skin and transferred with transparent adhesive tape onto a record sheet.
The filter was carefully lifted off the agar plates and transferred (with colonies facing up) to a pool of liquid nitrogen for 10 sec.
In Copenhagen she dances Odette-Odile in the "Swan Lake" that Mr. Martins staged there in 1996 and later transferred, with a few changes, to City Ballet.
The larvae hatched by the following day and were transferred with a fine sterile brush into the experimental tubes.
Remove from the oven and transfer with a palette knife.
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