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The session was immediately red flagged as medics rushed to his aide, and after being extricated from the cockpit and transferred by a stretcher to a waiting ambulance, he gave fans the thumbs up to show he was not seriously hurt.
At Drax pellets would be stored in domes, and transferred by a pneumatic conveyor system before grinding to dust for use.
The droplet images were recorded via a CCD camera and transferred by a framegrabber into a PC.
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After completing all m parts of job at the first stage, if the next stage machine is available, they are collected and transferred by an automatic transportation system.
Lysates corresponding to 500 000 cells were run on tris-glycine gels (Life Technologies) and transferred by an SV20-SDB semi-dry blotter (Sigma-Aldrich) to Hybond ECL membrane (GE Healthcare, Uppsala, Sweden).
The digested samples were submitted to electrophoresis on a 0.8% agarose gel and transferred by capillarity to a Hybond-N membrane (Amersham Biosciences).
Proteins (70 µg/lane) were resolved by SDS-PAGE and transferred by electroblotting onto a PVDF membrane, which was probed with a primary antibody for PEPCK Cayman Chemical Companyy, Ann Arbor, MI, USA) and actin (Sigma, St . Louis MO, USA).
Utilizing the PALM Laser-MicroBeam System, selected tumor areas containing 6000 10000 cells were microdissected and transferred by means of a laser puls to 15 µl DNA extraction buffer ATL (Qiagen) in the cap of a microfuge tube.
Sixteen small colonies were picked and transferred by CellCelector™ to a 96-well plate.
A 30 to 50 μg sample of protein was subjected to SDS-PAGE (NuPAGE, Invitrogen, Carlsbad, CA, USA), and transferred by electrophoresis to a polyvinylidene fluoride (PVDF) membrane.
Equal loading of each sample of protein (20 μg) was then subjected to SDS PAGE (NuPAGE, Invitrogen, Carlsbad, CA, USA) and transferred by electrophoresis to a PVDF membrane.
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