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Proteins were separated on a SDS-PAGE (4∼15% gradient Tris-HCL, Bio-Rad Laboratories) and transfered to a PVDF membrane using a semi-dry electrophoretic transfer device Bio-Rad Laboratoriess) and the 3-buffer system (Millipore, Billerica, MA).
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Chop coarsely and transfer to a bowl.
Drain it and transfer to a plate to cool.
Mix well and transfer to a serving dish.
Drain the beans well and transfer to a salad bowl.
Drain well and transfer to a small saucepan.
Season well and transfer to a tray or bowl.
Mix well and transfer to a serving bowl.
When the water returns to a boil, drain squid thoroughly and transfer to a mixing bowl.
Olivia was put on a life-support machine and transferred to a private Mexican hospital.
Season with salt to taste and transfer to a serving platter.
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