Sentence examples for and transcribed using the from inspiring English sources
gRNA-1 and gRNA-2 (Table S2) were cloned into the pDR274 vector (Addgene) and transcribed using the MEGAshortscript T7 kit (Life Technologies) following the manufacturer's instruction.
For RNA injection, plasmids were linearized with NotI and transcribed using the SP6 mMessage Machine kit (Ambion).
The corresponding cDNAs were purchased from ATCC, incorporated into expression vectors, amplified in E. coli and transcribed using the MEGAscript kit (Ambion Inc., TX).
The cRNA product was reamplified, cleaned (QIAquick PCR Purification Kit; Qiagen, Valencia, CA), and transcribed using the Riboprobe In Vitro Transcription System (Promega Corp., Madison, WI) according to standard protocol.
Total RNA was extracted using TRI reagent (Ambion) 48 h post-transfection, treated with DNase I (DNA-free™; Ambion, USA) and transcribed using the Moloney murine leukaemia virus reverse transcriptase (Promega, USA) and oligo-d(T 15 according to the manufacturers' recommendations.
The riboprobe was generated by cloning the FGFR3IIIS PCR product amplified from MCF7 cells into the pCR®2.1-TOPO vector (Invitrogen, Leek, Netherlands) and transcribing using the Riboprobe® in vitro transcription system (Promega, Southampton, UK).
RNA was isolated from control and MeJA-treated leaves and reverse transcribed using the ImProm-II Reverse Transcription System (Promega, Madison, WI, USA).
The primer tagged dsRNA was purified using QIAprep Spin Miniprep Kit QIAGENN) and reverse transcribed using the PC2 primer [14] and Superscript III) (Invitrogen).
Briefly, 80,000 MEF cells (WT, WT expressing the Flag-Pol β transgene and Pol β KO) were lysed and reverse transcribed using the Applied Biosystems Taqman® Gene Expression Cells-to-CT™ Cells-to-CT™ Cells-to-CT™ Kit
Total RNA was extracted using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands) and reverse transcribed using the SuperScript III reverse transcription system (Life technologies).
Myofibre fragments were removed by passing through a 40 µm cell strainer (BD Falcon), and satellite cells collected by centrifugation, washed twice with PBS, and total RNA was isolated (RNeasy; Qiagen) and reverse transcribed using the Quanti-Tect kit (Qiagen).
