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In fact, DNA methylation in benign breast epithelial cells has been related to a personal history of benign or malignant breast disease and to predicted breast cancer risk in two independent studies [ 19, 20].
In silico computer programs were used to find homologous DNA sequences in human and chimpanzee genomes, generate random sequences, determine DNA sequence alignments, sequence comparisons and nucleotide repeat copies, and to predicted DNA secondary structures.
BLAST algorithms (standalone BLAST version 2.2.15; TBLASTN and BLASTP, [ 93]) were used to compare sequences from the gene lists to X. laevis GeneChip® Xl-PSID consensus sequences [ 61] and to predicted proteins from the X. tropicalis genome assembly (4.1; proteins.Xentr4.fasta.gz, Xt4.1 predicted proteins [ 94, 95]).
The haplodiploid genetic system and extreme multiple mating by the queen both lead to differential matrigene patrigene relatedness among workers and to predicted matrigene patrigene conflict over phenotypes associated with nepotistic rearing of new queens, sex allocation, colony fission, nestmate recognition, and worker reproduction (Haig 2002; Queller 2003; Dobata and Tsuji 2012; Kronauer 2008).
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