For wheat, reverse genetics was not so "easy", and whilst stable transformation with RNAi constructs was being developed it was, and remains, a relatively time consuming methodology, which had not really addressed many pathogenic interactions.
Sequencing genomic DNA using primers identified from ESTs proved to be an effective but time consuming methodology of SNP identification in rainbow trout, therefore not suitable for high throughput SNP discovery.
The need to strictly follow this sometimes costly and time-consuming methodology has been questioned [30, 31].
Kinematic techniques have been used in pigs to study the effect of different flooring types on locomotion [ 10, 11] and to quantify lameness in sows [ 12], although this rather complicated, expensive and time-consuming methodology is unlikely to be extrapolated to a practical situation.
The latter finding is of particular important, since it supports the notion that EPCs obtained by labor intensive and time consuming cell culture methodology, thus exhibiting several functional properties of endothelial cells, are indeed related to vascular biology, and are not monocytic cells that may also be involved in inflammation.
Nevertheless, it has been revealed that the methodology for stage 1 of the RAP is laborious and time consuming.
However, the methodology of AFLP experiments and post-run data analysis are complex and time consuming compared with other markers [ 36, 37].
Such methodology can be used for any other reduced model problem that include multiple parameters and time consuming simulations.
Those are costly and time consuming.
The methodology to create such law is detailed and can be applied to any reduced model problem including multi parameters and time consuming simulations.
