The algorithm consists of a simple preprocessing stage and two steps of video inpainting.
To eliminate the effects of acceleration and deceleration, we used 10 intermediate steps of a trial (five steps of left foot and five steps of right foot), excluding at least the first three steps and the last three steps.
A minor excavation in 1995 found the footings and two steps of a stone stairway that provided access to the Great Hall of the tower.
Productivity was improved by stepwise increase of the methotrexate (MTX) concentration and two steps of subcloning by limiting dilution.
As shown in detail in Fig. 1B, we then moved the CASM down every 2 min, between the 9th and 16th depolarisations, in six steps of 20 μm and two steps of 10 μm.
Using Baron and Kenny's mediation model [ 26], two stages and four steps of data analysis were used during data interpretation.
Tissues were gradually dehydrated in ethanol series (75%, 90 % and 100) for 1 h each, and then the ethanol was gradually replaced with histoclear (Gadot) in five steps of 1:3, 1:1, 3:1 and two steps of pure histoclear (1 h each).
Samples had then been removed from each slice and subjected to tissue processing (dehydration, clearing and impregnation) on an automated instrument (Tissue-Tek VIP 5 Jr., Sakura Finetek Japan, Tokyo, Japan) that employed seven steps of 100% ethanol, three steps of xylene, and four steps of paraffin, with 8 hours at each step.
Previously, we established a purification procedure involving ammonium sulfate precipitation to enrich the Mediator-containing fraction and two steps of affinity chromatography using IgG and Ni-NTA columns.
At the end of the PCR, the reactions are combined and after three steps of denaturation, renaturation and DpnI digestion of methylated parental plasmid DNA, the products are directly transformed into host cells.
