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This tertiary structure is functionally relevant (and therefore constrained) because it defines the position of the catalytic cysteine residue with respect to the E2 enzyme and the ubiquitination substrate during the ligation process (Verdecia et al. 2003).
It has previously been shown that evolutionarily older proteins have higher degree [ 32] and that loss and gain of protein-protein interaction sites are driven by evolutionary mechanisms [ 33] and therefore constrained by positive (Darwinian) and negative (purifying) selection.
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Specifically, each unit cell contains one PA nanofiber, which is modeled as a cylindrical rod strongly adhered to the surrounding polymer in order to prevent slippage at the PA-polymer interface and therefore constraining the matrix during contraction (Fig. 4c).
Other useful features include marble veining, which is usually highly directional and therefore constrains the space of possible matches, the presence of straight borders and clamp holes, which denote fragments lying at the edges of slabs, and of course the continuity between fragments of the incisions themselves.
This is due to the implementation of a mechanism that ensures that each division manager reveals the true costs to the headquarters, and therefore constrains the possible range of transfer prices.
Moreover, because the plants in our experiment were grown in rich soil and under optimal growth conditions, it is less likely that resources would limit growth or reproduction and, therefore, constrain allocation to defence (sensu [39]).
It also specifies selected ARV manufacturers and therefore constrains use of local ARV production which Uganda started in 2008 [ 29].
This would also help clarify whether it is a reduction in oestrogen producing severe endocrine symptoms that interferes with attending to stimuli and therefore constrains memory processing, or chemotherapy treatment per se.
Further studies will help clarify if additional WNV adaptation to a vector population could lead to increased virulence in these vectors and therefore constrain the evolution of more efficient strains.
However, on this account, it becomes harder to retain the conservative project that looks for the natural phenomena that fall under, and are therefore constrained by, our folk concepts of health and disease.
However, such schemes only exploit the cooperation among the sources and are therefore constrained by the number of sources.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com