At 138 minutes, with a lone weeping fiancée briefly glimpsed on shore (and thereafter in a black-and-white photograph), "K-19" acquits itself honorably on both counts, though the director, Kathryn Bigelow, is a woman.
The daddy's a second-generation Italian immigrant who buggers off, never to be seen again, leaving his teenaged wife, an Austrian au pair, to bring up baby with her in-laws and, thereafter, in a one-room flat with a blanket strung across the middle on a washing line.
Because New York State had not yet legalized same-sex marriage, this couple too was legally married in Toronto, Canada, and thereafter in a religious ceremony in Rhode Island.
Lyophilized Aβ(1 42) was dissolved in trifluoroacetic acid at a concentration of 1.0 mM, left to stand at room temperature for 1 h and dried under a stream of nitrogen and, thereafter, in a vacuum (1 mm Hg) for 15 min. The peptide was then redissolved in hexafluoroisopropanol at a concentration of 1.0 mM and, after 1 h of incubation at room temperature, dried as described above [39].
Within the JU-LMU Link German students have the possibility to participate in the community based training program (CBTP) and thereafter in a clinical rotation in a self-chosen clinical field.
In short, the screws were incubated first in a solution of 2 mg/ml plasminogen free fibrinogen for 10 minutes (Haemochrome Diagnostica, Sweden), and thereafter in a solution of 1 μg/ml ZOL for 10 minutes [ 33].
The survey was developed and validated by consensus by a group of anesthesiologists and clinical epidemiologists, and tested thereafter in a small group of anesthesiologists.
Single intact flexor digitorium brevis (FDB) fibres (fast-twitch, type II) were obtained by dissection and thereafter mounted in a chamber between a force transducer and an adjustable holder.
When incubated at 4 °C, in comparison with the initial diameter at day 0, the diameter of the nanoliposomes increased by 14.8% on day 5 and varied thereafter in a smaller extent of change in the diameter.
Tissue specimens were fixed at room temperature overnight with 4% formaldehyde in 0.1 M phosphate buffer solution (pH 7.3), washed for 4 6 hours in 0.1 M phosphate buffer and stored thereafter in a 50% EtOH solution at 4°C.
After 2 weeks, the butterflies were transferred to a 50-ml plastic cup and thereafter housed in a cold room maintained at 10 °C until the onset of the experiment.
