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Other gene clusters did not show a gene cluster specific profile shape (see Figure 2) and the two template profiles were not found among them, indicating a different control.
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Using the PTM calculation function of MeV software, we determined the correlation between the expression pattern of each gene and those of the two template genes.
Extract the centroids c i and c j of the two template shapes.
For each ordered template shape pair M i, M j, its R-histogram is computed through the following steps: (1) Extract the centroids c i and c j of the two template shapes.
In both structures, all the zinc atoms lie in essentially the same plane, and in c- P8⋅(the 2, two templatelate units overlap at the center.
Apart from hRHO, the differences in sequence similarity between each target GPCR and the five template structures are small, ranging from 3% to 11% (Table 3)–see materials and methods for a definition of percentage of sequence similarity.
The conservation of the starch biosynthesis process among the six plant species, including cassava and the five template plants, were observed at both molecular sequence and pathway levels.
Like-wise, when restricting the comparisons to individual helices, the differences in sequence similarity between each target GPCR and the five templates structures are also small (Table 4 and supporting ), although the sequence similarity values of these helical regions tend to be higher.
The average pairwise sequence identities between Populus MIPs and the three templates range from 21 to 45%.
The different fluorescence signals of T +1)2-AP in the four binary complexes are somewhat surprising and imply variability in the interaction between the T +1) base and the four templating bases.
Five different sequences were uncovered from the two-template PCR reaction; one copy of each of the two templates and three recombinant sequences.
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