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This list of miRNAs was then compared with that of the miRNAs predicted by miRDeep and the sequences present in the blast list but absent in the miRDeep list were considered false negatives.
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Trees are constructed solely based on the homologous positions after selecting the conserved positions and removing the gaps from the alignment The minimal number of conserved positions used to construct the tree is 58 amino acids (except for one xylanase that was much shorter but was based a on manual check of the alignment and of the sequences present).
For the circular representation and comparison of the sequences present in the communities against known bacterial genomes, we used the CGView server (Grant and Stothard, 2008).
NaGIR2 represents the splicing-ribozyme domain of Nae.S516 and thus corresponds to the sequences present in a prototype group I intron such as the Tetrahymena intron.
Therefore, PRMT5 and PRMT4 are likely to regulate pre-mRNA splicing, at least in part, by contributing to stabilizing weak RNA-RNA interactions between donor splice sites that deviate from the consensus sequence, and the sequence present in the U1 SnRNA that is complementary to the consensus donor splice-site sequence.
First, the array designs target only the sequences present in the Nipponbare genome, and therefore we could not detect the sequences present in other rice cultivars but absent from Nipponbare.
The MspI-3 and the MspI-4 sequences presented no significant similarity (BLASTN and BLASTX) with any of the sequences present in Genbank.
The mutagenesis primers were obtained from Eurofins MWG Operon (Ebersberg, Germany) and contained the sequences presented in Table 1.
Discrepancies between the two plastid assemblies were corrected by mapping the Sanger and 454 reads against the two sequences using BLASTN (e-value = 1 × 10−, dust = no) and taking the sequence present in the majority of mapped reads.
For instance, a user can specify 'receptor' as a keyword and restrict the results to the sequences presenting positive results for transmembrane domains and signal peptide.
Also note that in some cases there are slight differences between the sequences presented here and the sequences with the provided ENSEMBL IDs.
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