Sentence examples for and the second strands from inspiring English sources

From these fragments, the first strands were synthesized using random hexamer primer and the second strands of cDNA were then synthesized.

The mRNA was used as a template for first-strand cDNA synthesis using N6 random primers and MMLV reverse transcriptase (TaKaRa, Dalian, China) and the second strands were synthesized using Secondary Strand cDNA synthesis enzyme mixtures (Autolab, Beijing, China).

The RNA templates were then removed by RNaseH and the second strand was synthesized using DNA polymerase I to generate double-stranded cDNA fragments.

We shall call one strand policy analysis and the second strand political economy.[2] These two strands may be differentiated along a number of dimensions.

NSR hexamers were synthesized with a 5′-amplification annealing site for the first-strand (5′-TCCGATCTCTN- NSR reverse complement)-3′) and the second strand (5′-TCCGATCTCTN- NSR)-3′) primers.

The RNA strand was digested with RNase H and the second strand cDNA was further synthesized with DNA polymerase I.

An oligo-dT primer was used for first-strand synthesis and the second strand was random-primed.

The first and the second strand cDNAs were synthesized and end repaired.

The first strand of cDNA was then synthesized by priming with random hexamer, and the second strand was generated with buffer, dNTPs, RNase H and DNA polymerase I.

Second, IdeS cleaves the first strand rapidly and the second strand slowly, which presents a practical window for the isolation of homogeneous scIgG-T for in vitro and in vivo studies [ 14, 18, 40].

The first strand cDNA synthesis was carried out using reverse transcriptase (Invitrogen) with random hexamer primers, and the second strand using RNase H (Invitrogen) and DNA polymerase I (New England BioLabs).