The phrase "and the second strand" is correct and usable in written English.
It can be used when discussing multiple components or aspects of a topic, particularly when referring to a sequence or series.
Example: "In our analysis, we will focus on the first strand of the argument, and the second strand will be addressed in the following section."
Alternatives: "and the subsequent strand" or "as well as the second strand".
Then the first cDNA strand was synthesized using the mRNA fragments as templates, and the second strand was generated using a SuperScript Double-Stranded cDNA Synthesis kit (Invitrogen, Camarillo, CA), purified via magnetic beads, the ends repaired and a single nucleotide A (adenine) added to the 3′ ends.
The RNA templates were then removed by RNaseH and the second strand was synthesized using DNA polymerase I to generate double-stranded cDNA fragments.
One is looking specifically at the actions of Savile, and the second strand concerns allegations against "Savile and others".
One is looking specifically at the actions of Jimmy Savile and the second strand concerns allegations against "Savile and others".
It has three strands - one is looking specifically at the actions of Savile and the second strand concerns allegations against "Savile and others".
We shall call one strand policy analysis and the second strand political economy.[2] These two strands may be differentiated along a number of dimensions.
From these fragments, the first strands were synthesized using random hexamer primer and the second strands of cDNA were then synthesized.
The mRNA was used as a template for first-strand cDNA synthesis using N6 random primers and MMLV reverse transcriptase (TaKaRa, Dalian, China) and the second strands were synthesized using Secondary Strand cDNA synthesis enzyme mixtures (Autolab, Beijing, China).
First strand was created using reverse transcriptase and random primers; the second strand was then synthesized to generate double-stranded cDNA.
Second strand cDNA was synthesized with a dNTP mix containing dUTP, and after adapter ligation the second strand cDNA was digested to allow for strand-specific sequencing.
These fragments were used to synthesize the dsDNA using random hexamer primer for the first strand and DNA polymerase I for the second strand.
