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The enzymes' redox potential oscillates between 300 and 800 mV; these fluctuations depend on different factors such Cu ligands, distance among Cu, and the second sphere of amino acids in the active site.
For each of the CMM-scanned liners, 2 observers independently aligned the 2 spheres such that one sphere was positioned in the original position of the femoral head (pre-wear position) and the second sphere was positioned at the location of maximum wear.
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The coordination of these zincs is tetrahedral, and the distances are typical for ADHs; however, the catalytic zincs in subunits A and C ligate TFE, with Glu-67 OE2 in the second sphere, and the zincs in subunits B and D ligate Glu-67 OE2, with TFE in the second sphere (Table 5S of the Supporting Information).
They encounter the second fiend, Sei-ryu; they defeat him and recover half of the second sphere.
Most metal-containing oxygenases utilize amino acids in the second sphere and beyond to regulate the O2 activation reaction.
Mutations in the iron-coordinating His residues, the second sphere Glu residues, and even a third sphere of residues that form hydrogen bonding interactions with the second-sphere Glu residues either abolish or greatly reduce RPE65 activity.
An administrator was appointed to manage and monitor the financial affairs of the local government, by the second sphere of government, known as the Provincial Administration in South Africa.
The second sphere naturally made her the protectress of women in childbirth, and she bore the title of Eileithyia, the birth goddess, at Argos and Athens.
Thus, control of the second sphere allows the enzyme to design a specific catalyst that gives only one of the four potential types of products.
The second sphere provided the comparison stimulus for the time discrimination.
The structure of FeSOD and MnSOD separates metal binding by conserved first-sphere residues that also provide a baseline for the value of E°, from strong differential redox tuning delivered by the second sphere by virtue of an exceptionally tunable ligand: OH /H2O.
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