Sentence examples for and the second reverse from inspiring English sources

The primers that have been used are the forward primer 3-DTAclv; And the reverse primers: (in this order, when the PCR product of the forward and the first reverse primer used as template for the reaction with the forward and the second reverse primer, and so on): 4-DTAclv, 5-DTAclv and 6-DTAclv.

Primers for amplification of the 35S promoter and 5? nptII region are as follows: forward primer: 5 -CATTACATCACCCATAATAAATACTTTCTC-3?; the first reverse primer: 5 -CATTACATCACCCATAATAAATACTTTCTC-3?; and the second reverse primer: 5 -CATTACATCACCCATAATAAATACTTTCTC-33?

The primers for the amplification of the 35S promoter and 5′ nptII region are as follows: forward primer 5′-CAT TAC ATC ACC CAT AAT AAA TAC TTT CTC-3′, the first reverse primer 5′-GAA TAG AGA GAA AGA TAT ATT TTT TAA GAT-3′ and the second reverse primer 5′-GTA ATA GAG ATT GGA GTT TTT AAG AAA GTA G-3′.

After 1 h at 42°C, 2 μl of RT reaction was used for PCR amplification with the second reverse primer and the corresponding forward primer (see Additional file 7) in a final volume of 20 μl.

Differential scanning calorimetry measurement showed that both the critical temperature and the endothermic heat of the first reverse transformation of the deformed martensite were increased as compared to an undeformed specimen, indicating a stabilisation effect to the reoriented martensite.

Andrea Petkovic delivered the decisive blow, beating Melanie Oudin, 6-2, 6-3, in the first reverse singles, and Sabine Lisicki then beat Christina McHale, 6-3, 6-4.

Then the window shifts based on the previous (first) reverse primer and the next (second) forward primer is designed from the upstream region of the first reverse primer.

For while the first reverses Citizens Unitedd and the third is intended to protect media corporations and non-profits, the second aims to broaden the scope of permissible regulation on political contributions and expenditures.

The PCR product was digested with BamHI and XhoI (restriction sites underlined in the forward and second reverse primer, respectively) and subcloned into the pFASTBAC1 vector (Invitrogen) cut with the same restriction enzymes.

In the first of these, all studies have twice the number of subjects in the treatment arm (q=1/3) and the second is reversed with all studies having twice the number of subjects in the control arm (q=2/3).

To obtain best possible coverage of the urine proteome for relative quantitation the MS analysis was preceded by a two dimensional tryptic peptides separation, the first dimension being isoelectrofocusing (IEF) and the second dimension – reversed phase liquid chromatography (LC).