Sentence examples for and the second primary from inspiring English sources
In the presence of 2 1 internal resonance, both the first and the second primary resonances are examined in detail.
The slide was washed again and the second primary monoclonal antibody Pfg377 (1∶400 dilution in PBS-1% BSA) was added for 1 hour.
The sequential ChIP studies were performed as previously reported [10] and following the sequential immunoprecipitation studies described, using the first primary antibody (anti-EGFR) and the second primary antibody (anti-Src).
After several washes, the tissue was blocked again in PBS 10% FBS, 0.2% Triton X-100 and the second primary antibody PHF-1 (monoclonal, 1∶200; 1 h) or a mixture of anti-Aβ 4G8/6E10 (1∶1000 each, 1 h) was added, followed by incubation with alkaline phosphatase labeled horse anti-mouse IgG (1∶500, 1 h; Vector Laboratories).
The mean interval between the index primary and the second primary was 117±124.0 months, with a median of 76.5 months.
Next, the sections were microwaved to eliminate endogenous HRP activity and the second primary antibody (rabbit polyclonal anti-cyclinD1; Santa-Cruz sc-8396 (Santa Cruz Biotechnology, Inc. Heidelberg, Germany)) was used at a 1 500 dilution at 4°C overnight.
In the case of double labeling, the following combinations of antibodies were used for this study: HIF-1 α/VEGF, HIF-1 α/Ki-67, HIF-1 α/cyclin D and HIF-1 α/cyclin E, PC3 cells were incubated with the first primary antibody overnight, followed by the first secondary antibody, and then the second primary antibody and the second secondary antibody.
MACH 2 HRP polymer detection system and VIP substrate (Vector Laboratories, Burlingame, CA, USA) were used to tag and stain the second primary antibody purple.
Data were provided from each cancer registry on all initial primary cancers including age and sex of the subject, diagnosis and date of the first primary, follow-up for mortality and date and diagnosis of the second primary, if any.
The relative difference between the SUVs of these tumours (∆SUV) was determined and compared between the second primary group and metastatic disease group.
The first primary antibody was developed with an HRP-conjugated secondary antibody or biotin/streptavidin, and diaminobenzidine (brown), followed by the second primary antibody and then the alkaline phosphatase-labeled secondary and NBT (blue).
