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Fixed cells were incubated in PBS+FBS+γ-globulin block solution for 10 minutes, and the same solution was used as the diluent for primary and secondary antibodies.
This was achieved by an initial 24 h wash at 4°C in 1 ml of a blocking solution containing 0.5% Triton-X-100 on a shaking platform and the same solution was used for all subsequent labelling and wash steps.
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A prime subnetwork of Γ is a subset of Γ which contains no redundant constraints and has the same solution set as Γ.
Although deviating in tone and plot from Terry Gilliam's 1995 steam punk classic, the series begins with the same event — an international plague that decimates the world's population — and offers the same solution: time travel.
The algorithm converged in all tested conditions, and onto the same solution when run repeatedly.
Deterministic algorithms in general follow more rigorous procedures repeating the same path every time and providing the same solution in different runs.
The same principle holds for sound, and the same solutions are in some ways possible.
The merit of the assumption is that it leads to the same differential equations of equilibrium and the same solutions as those for shallow circular arches.
Although GAMS and CPLEX are useful to solve the mixed-integer model, we solve a small case of this problem and the same solutions are obtained.
Gels were then stained with 0.5% Coomassie blue in 25% methanol and 10% acetic acid, and destained in the same solution without Coomassie blue.
Brains were removed and postfixed in the same solution for four hours and cryoprotected in 30% sucrose overnight.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com