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Due to the sophisticated technique, the high costs for mallein and mAbs and the restricted availability of these mAbs, the cELISAs will not be available for routine laboratories in the future.
Owing to methodological limitations and the restricted availability of pure mitochondrial RNA, primer extension remains the most practicable method for analyzing mammalian systems (17, 19, 30).
The missing evidence of nosocomial respiratory tract infections as defined by the CDC criteria during the study period could be explained by the absence of an intensive-care unit with invasive ventilation procedures [ 22, 23] and the restricted availability of the X-ray machine.
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Today, wax esters are harvested from plants and animal tissues, or generated by chemical synthesis using fossil sources, and this is considered to be the main limitation for their application due to the restricted availability and high costs of existing sources [ 2, 4].
As a consequence, H2S production and the activity of sulfide-oxidizing bacteria will also be low due to the restricted availability of oxygen and nitrate deeper in the sediment.
Complications during delivery as a result of macrosomia can lead to additional hazards to the mother and newborn in resource scarce settings as compared to resource rich settings because of the restricted availability of emergency obstetric and other essential care [ 23].
The specific difficulties to be faced are the restricted availability of classrooms and the increased flexibility of the students' choices of courses, which makes the problem very tight.
Porter has also pointed to the restricted availability of England-based players and the miserable winter endured by the national side.
The derivation and use of human ES cells are limited by the restricted availability of human embryos, low efficiency of isolation of human ES cells, the potential for immune rejection of non-matched tissue (West et al., 2010), and by ethical concerns surrounding the destruction of human embryos for the purpose of cell isolation.
Reasons for underpowered studies may be the restricted availability of model animals, or the drive and objective of ethical committees and regulatory authorities to minimize the number of animals permitted in a study [ 76].
However, the restricted availability of mapped distributions for climatic envelope modelling and of long-term population monitoring data may restrict the scope for such developments in the immediate future.
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