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The results provided direct evidence for Cu PICT in the soil, and the quantification method developed in this study has the potential to be used as a molecular assay for soil Cu pollution.
The accuracy of the quantification was dependent on the SBR, the size of the spheres and the quantification method used.
Briefly, qRT-PCR was performed according to the MIQE guidelines 28 and the quantification method was done according to Livak and Schmittgen 29.
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We validated the coexistence of the four variants by PCR and, adjusting the quantification method for a four-variant case, were able to measure the abundances of each inversion event separately.
Skeleton processing, graph processing, and the quantification methods are implemented in independent modules so any improvement or extension to any of these processes can be made very easily.
Those ions were chosen for the MRM mode for VNT and IS in the quantification method (Fig. 2).
Based on the number of tissue samples and the resolution of the quantification method, we defined a difference in miRNA abundance by ±50% from Control, corresponding to ±0.58 on the log2RQ scale, as up- and down-regulation, respectively.
Notably, after log transformation and batch effect correction, the quantification method even explains slightly more of the variation than the tissue, whereas all other factors are essentially uninformative.
A recent study [ 6] compared different mapping and quantifications pipelines applied to the same (experimental and simulated) data, concluding that the quantification method introduces more variation than the alignment methods.
Infarct size and myocardial quantification vary depending on the quantification method used.
See 'Materials and methods' for a description of the quantification method.
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