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The sequence identity between the helix 7 of the template and the corresponding helix of the target is only 6%, and the global sequence identity is only 27%.
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EMBOSS Needle [ 43] was used to perform and visualize the global sequence alignment between exFeLV and enFeLV.
This methodology allows to perform comparative analysis between the behavior of the four nucleotides and of the global sequence.
The best hit in the reference database is identified for each read, and if the global sequence identity is greater than or equal to a user-selected level, e.g. 97%, the read is assigned to the corresponding species-level taxon.
> The histograms of the distance sequences were computed for each nucleotide and also for the global sequence.
In spite of the global sequence and structural similarities between groups IV and V, the differences at both C-terminal domains and thioredoxin active domains [ 20] did not allow any inference on their origin and evolutionary relationships.
In order to gain further insights on how genes have diverged between the indigenous and invasive whiteflies, we compared the global sequence divergence between the transcriptomes of Asia II 3 and the invasive species MEAM1 and MED.
Analysis of the global sequence similarity and calculated percentage identity demonstrated that both Omp3 and Omp7 revealed considerable similarity to reference proteins (Fig. 2).
Looking at data for all 16,752 probe sets (irrespective of their changes in gene expression) we saw an increase in the number of homologues between the worm and the yeast, which corresponds to the global sequence similarities between these genomes.
Edges represent the global sequence similarity.
The protein sequence similarity was converted from the global sequence distance obtained by ClustalO [14].
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