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The extraction procedure was repeated twice and the extracts were combined and evaporated under reduced pressure, to give a dark brown residue with a yield of 9.8%%.
The tryptic peptides were extracted from gel pieces with extraction buffer (70%ACN/5%% formic acid) and the extracts were dried down in a vacuum centrifuge.
This extraction procedure was repeated three times and the extracts were combined.
A fast and quantitative pressurized fluid extraction procedure was optimized using experimental design and the extracts were analyzed without any further clean-up step.
Briefly, liquid-liquid extraction and column chromatography cleanup were used, and the extracts were analyzed by gas chromatography with electron capture detection using an internal standard.
The extraction and desorption operations were enabled more conveniently by magnetic separation and the extracts were analyzed by high-performance liquid chromatography coupled with ultraviolet and fluorescence detection.
The tryptic peptides were extracted from gel pieces with extraction buffer (75% acetonitrile / 5% formic acid), and the extracts were dried out in a vacuum centrifuge.
The extraction process was repeated three times to exhaustively extract the same plant material, and the extracts were combined.
After extraction, the vessels were allowed to cool to room temperature, and the extracts were filtered and stored in a refrigerator.
The extract was centrifuged at room temperature for 10 min at 4000g and the extracts were combined.
Brachiaria decumbens shoots was extracted as described above and the extracts were concentrated at 40 °C in vacuo to produce an aqueous residue.
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