Samples were analyzed on a BD LSR II flow cytometer (BD Biosciences) and the data were analyzed using FlowJo software (TreeStar).
The FITC, PE and APC channels were used to detect the emission of the conjugated surface antigens and the data were analyzed using Cyflogic (CyFLo Ltd ,Turku, Finland).
The experimental manipulations were induced using video clips and the data were analyzed using a repeated measures MANOVA procedure.
Stained nuclei were analyzed with a fluorescence-activated cell sorter (FACS) Vantage (Becton-Dickinson) and the data were analyzed using a Mod-Fit cell cycle analysis program (Becton-Dickinson).
Statistical analyses were performed in SPSS 11.0 and the data were analyzed using one-way ANOVA.
The cells were subjected to flow cytometry, and the data were analyzed using FlowJo version 7.6.1.
All statistical analyses were performed using SPSS 10.0, and the data were analyzed using one-way ANOVA.
The specific surface area of TCAC was measured via a nitrogen adsorption isotherm using an ASAP 2010 Micromeritics instrument and the data were analyzed using the Micromeritics software.
The level of significance was predetermined at 5%% (p = 0.05), and the data were analyzed using the BioEstat 5.0 software (BioEstat, Belém, Brazil).
The three points flexural strength was performed 24 h after cementation and the data were analyzed using one-way ANOVA and Tukey's tests (p-value = 0.05).
Fluorescence was detected using a FACS AriaTM and the data were analyzed using the CBA software (BD Bioscience).
