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In view of these results, international guidelines should help to standardize the pre-analytical phase, the staining techniques and the counting methods.
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Grain detection and the counting method were compared to two academic methods as well as to ground truth and P-TRAP outperformed the other methods.
The differences between individual mice were significant (p = 0.01) and highly significant between the counting methods (p < 0.001).
We have proposed that the reasons for these contradictory results may be the use of different antibodies to endothelial cells (ECs), the counting methods and the staining procedures including antigen retrieval techniques (Wang et al, 1994).
It is interesting to note a prolonged increase in LB counts irrespective of the cessation of probiotic intervention, which may be explained by the ability of Lactobacillus paracasei SD1 to be retained in the oral cavity, as previously reported [ 22] and by the counting method for total LB on MRS agar in this study, rather than the specific quantification of Lactobacillus paracasei SD1.
Further details and the rationale for the counting method have been presented previously [ 28].
We did not observe any correlation between the numbers of viable cells and the type of medium used for thawing, the counting method, and the duration of the resting phase [8 centers included a short resting phase in their protocol (2 6 h) and 8 centers used an overnight resting (14 20 h)].
The collector used with a recirculating aquaculture system and the count method is a promising technique for accurate determination of FCR in aquaculture research.
Asselin Charles, a spokesman for Prime Minister Jacques-Edouard Alexis, said outside countries had misunderstood the counting method and that the results could not be changed because elections are the responsibility of the Provisional Electoral Council.
In this latter section, the counting method and the decisional mechanism are explained separately.
Coefficient of Error represents a measure of the variance of the estimate from counting; which includes both the biological variation and the error introduced by the counting method.
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