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The pGEM backbone was excised from pHTK [34] by digesting with Nar I- Not I, and pGEM and the components described above were assembled into the pSTCII-GFP plasmid through several cloning and subcloning steps.
In addition, the workflow and the components described in this paper can be used in any domain, so they are not restricted to agriculture; one can simply use another thesaurus to annotate web resources and populate the Crawler Database.
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Finally, S. coelicolor was cultured in a fermenter in minimal medium with EFBH as a carbon source and addition of the components described above.
The treatments were the Simulation of Clinical Cases and the Edugame components described in the paper.
Importantly, these silencing mechanisms involve factors regulating H3K9me3 and its cognate-binding protein, HPL-2, and also MES-4 and the MES/PRC2 components described above [ 89].
The results of Phase 1 of the current study will determine the periodicity and other components (described in the phase 1) of the intensive phase.> A psychologist coach is in charge of the intensive phase, which includes motivational, coaching psychology and group development techniques, and evidence based information.
In terms of the training protocol, each session began with Tai Ji Quan-based movement warm-up exercises (5 10 minutes), followed by teaching and practicing the core components (described in the following section) (40 45 minutes), and ending with a simple set of breathing exercises (3 5 minutes).
The PTs providing usual care will receive a two-hour training course in the rationale and treatment components described in the LBP guidelines issued by the Royal Dutch College for Physical Therapy (KNGF) [ 27, 28].
Present organizations were advised to take advantage of this project support (project implementation manual, downloads of intervention materials, contacts), to form a local steering committee independently, and to implement '10,000 Steps' according to the guidelines and intervention components described in the project implementation manual.
21 The platform and its components described below provide the research community with a high-performance environment for the crowd-verification of biological network models.
The core and additional components described above will be used to encourage behaviour change and to reinforce knowledge about the potential benefits of PA.
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