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The full-length ORF of PhaNPR1 was cloned, and the complete sequence was determined.
We obtained almost the complete sequence of exon one (except 186 bp in the 5' end of the exon), and the complete sequence of exon three (Table S3).
The PCR product was cloned into the pENTR/D-TOPO Gateway entry vector according to the manufacturer's instructions (Invitrogen) and the complete sequence was verified by DNA sequencing.
BLASTn searches were undertaken using the nucleotide sequences of these reference genes and the complete sequence of Sinbad (Table 1).
The exact location of those residues in our RASSL construct, and the complete sequence of all RASSL variants can be found on our web site.
The Smed454 dataset can be freely accessed via a website, and the complete sequence data can be downloaded by anyone from there.
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From these genome-contigs, we designed synthetic oligodeoxynucleotide primers, carried out PCR amplification and nucleotide sequencing, and assembled the complete sequence for a novel, tightly organized, seven-gene hyqRBCEFGI operon (GenBank accession: FJ378904; Fig. 2).
For strain BAI3, we performed additional cloning and sequencing experiments to assemble the contigs and determine the complete sequence of an 82-kb region containing META-B.
Exploiting these results to design degenerate PCR primers, we amplified and cloned the complete sequence of armB, a PKS gene from the melleolide producer Armillaria mellea.
This presented an opportunity to further examine the genomic diversity within the A1 population, and therefore, the complete sequence of a F. tularensis subspecies tularensis A1 isolate NE061598 was determined.
These make it difficult to obtain a long contig sequence of the W chromosome, and thus the complete sequence of the W chromosome remains to be resolved.
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