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We show that by using stringent data processing requirements differential expression as determined by RNA-Seq is concordant with both the Affymetrix and Illumina platforms in more instances than it is concordant with only a single platform, and that instances of discordance with respect to direction of fold change were rare.
We show that by using stringent data processing requirements differential expression as determined by RNA-Seq is concordant with both the Affymetrix and Illumina platforms in more instances than it is concordant with only one, and that instances of conflicting directions of fold change are rare.
We conclude that neither the weak phenotypic manifestation of pathogenic mutations or erroneous description of benign polymorphisms as pathogenic mutations substantially contributes to our data and that instances when an ancestral state matches a human pathogenic mutation can generally be considered to be irreversible states.
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