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ORF1 started with an UUG (Leu) initiation codon 21 nucleotides downstream of the predicted 5'end and terminated with a UAA termination codon produced by alternative editing after 117 amino acids.
The side chain of this type of polyelectrolyte was incorporated by two quaternary ammonium groups and terminated with a cross-linkable unsaturated bond.
Much research has verified that tripeptides initiated with a branched-chain aliphatic amino acid residue and terminated with a proline have a strong antihypertensive activity in vivo.
The radiating cable used in this experiment was a RADIAFLEX® model RCF 12-50 J, manufactured by Radio Frequency Systems RFS (Hanover, Germany) [8] and terminated with a matched 50-Ω load to avoid any unwanted reflections.
Each parabola is initiated with a 1.8 g pull-up and terminated with a 1.8 g pull-out by the aircraft.
The reaction protocol included: 5 min at 95°C, followed by 45 cycles consisting of 10 sec at 95°C, 20 sec at 60°C and 20 sec at 72°C, and terminated with a melting curve analysis (from 60°C to 95°C) to check the specificity of the PCR product.
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The experimental setup is a varying width plate immersed in fluid and terminated with an acoustic black hole.
Each PCR was initiated with a denaturation step at 95°C for 5 min and terminated with an extension step at 72°C for 5 min. The PCR product was diluted 1 100 in distilled water and 1 µl of the dilution was mixed with 10 µl of formamide and 0.3 µl of GeneScan 500 LIZ Size Standard (Applied Biosystems, Warrington, UK), denatured at 98°C for 5 min and cooled on ice.
Protein coding regions invariably begin with an ATG start codon and terminate with a TAG/TAA/TGA stop codon.
Both synthetic genes are driven by the strong early/late vaccinia virus promoter mH5 and terminate with a vaccinia virus specific stop signal downstream of the coding region that is absent internally.
First, the model must be complete; it must begin with an ATG codon and terminate with a stop codon (TAA, TAG or TGA).
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