Sentence examples for and template in a from inspiring English sources

Reactions contained 7.5 µl 2× SYBR MasterMix (Qiagen), primer (100 nM), and template in a total volume of 15 µl.

Here, we experimentally test whether nest soil affects the odour label and template in a soil-nesting ant species, Camponotus aethiops.

The reaction mixture contained 1× Taq PCR buffer, 0.625 µmol/L primer ERIC1R (5′-ATGTAAGCTCCTGGGGATTCAC-3′), 1.9 mmol/L MgCl2, 50 µmol/L dNTPs, 0.6 U Taq polymerase, and template in a total volume of 20 μL.

Each reaction contained 12.5 μl SYBR green PCR mastermix (Applied Biosystems), 3.5 μl H2O, forward and reverse primers in a volume of 5 μl, and template in a volume of 4 μl.

The PCR mixture consisted of 10x buffer, 25 mM MgCl2, 25 mM dNTPs (TaKaRa, Japanese), ultrapure water, 10 mM upstream primer, 10 mM downstream primer, 50 × sybr, 5 u/ μL Taq enzyme, and template in a final volume of 25  μL.

August's research offers an automated program that enables computer programmers automatically to identify macro-viruses, which can infect data files, documents and templates in a computer system.

The other part was the rescoring time which calculated the DTW matching scores between a test segment (time marked by a phone arc on the phone lattice) and templates in a template clusters (specified by the PDTs of the phone unit).

A descriptive qualitative approach was undertaken using observations, semistructured interviews and template analysis in a thematic analysis framework.

Conventional DNA polymerases extend off double-stranded DNA (dsDNA) substrates, containing both primer and template strands, in a 5′ to 3′ direction.

These PCR products were mixed and used as template in a reaction with EAO47 and EAO50 to yield a ~1.6-kb spo13pr-wsc1 + fusion fragment.

The two amplicons were then purified, mixed and used as template in a third PCR reaction, with primers SAPA60 and SAPA61, to generate the PCR-spliced full-length product.