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11671_2014_2496_MOESM1_ESM.doc Additional file 1: Supporting method and supporting figures.
CD characterization and AFM statistical analysis is provided in Supporting File S1 and Supporting Figures S10 and S11.
Our experiments demonstrated that, in vitro, the triple helix of the collagen fragments has a decreased stability at 37°C and became fully denatured above 39°C (Supporting File S1 and Supporting Figures S4 and S5).
Such pronounced structural deformation of the collagen was statistically evaluated by measuring the area of denatured collagen molecules following interaction with MMP-9 (Figure 3D - additional details on the characteristics of denatured collagen images are given in Supporting File S1 and Supporting Figures S7, S8 and S9).
Experimental details and supporting figures.
Complete experimental details and supporting Figures S1 S3.
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A significant decrease in the sodium diffusion coefficient was indicated at x > ~1 in Na2−xV3O7 where the Na5 sites were almost empty, which agrees with the results of the AC impedance measurements (Supporting Section S3 and Supporting Figure S6).
Mimic-210 suppressed ISCU mRNA levels by approximately 60% (Figure 1C and Supporting Figure S2A).
Moreover, anti-210 partially reversed the hypoxic suppression of ISCU protein (Figure 1D and Supporting Figure S2D).
Under hypoxia, MCF7 and HCT116 cells showed a reduction in ISCU protein, and this was replicated with mimic-210 in normoxia (Figure 1D and Supporting Figure S2D).
The BPC6-ΔC fragment contains the entire N-terminus harboring the conserved coiled-coil domain (Figure 1 and Supporting Figure S4).
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