The asymmetric unit of the crystal contained four molecules that were highly similar to each other and superposed with an rmsd of 1.17 ± 0.50 Å in secondary structure matching with 165 ± 16 selected pairs.
E. coli SSADH was purified and crystallised in the presence of the reducing agent β-mercaptoethanol, and accordingly, the structure we report most closely resembles the active form of human SSADH (2w8o) and superposes with a root-mean-square deviation of 0.79 Å over 472 Cα (2w8o and Monomer A, Figure 3A and Figure S2).
The images are binarized and superposed with the minimum bounding box (green).
48 hours after transfection, FLAG-Gpr124 (A ) and HA-Reck (B ) fusion proteins were detected by indirect immunofluorescence and superposed with the endogenous GFP fluorescence of the Fz4-GFP fusion protein.
The structures of the two independent molecules of the asymmetric unit were similar and were superposed with an RMSD value of 0.56Å over 232 Cα atoms.
The frequency dependence of G′ and G′′ at different temperatures can be superposed with a horizontal shift factor and a small amplitude adjustment.
However, a (contrived) state of the pointer in which all its protons are localised in region A and all its neutrons in region B, superposed with a state in which the protons are in B and the neutrons are in A, would also trigger a collapse, but onto one of these very non-classical states.
The equivalent main-chain atoms in the N-terminal domains of BcDltA in adenylation conformation (PDB entry 3DHV) and BsDltA in thiolation conformation (PDB entry 3E7W) are superposed with a root-mean-deviation of 0.97 Å, and the deviation for the C-terminal domain was 1.00 Å.
The two proteins share a similar structural fold given that 107 Cα atoms of total 116 Cα atoms across the length of the proteins could be superposed with a root mean square deviation (RMSD) value of 1.2 Å and a sequence identity of ~ 28%.
Most likely, these bands are superposed with a more intense band characteristic for a Fe OH bond in spinel.
