Sentence examples for and subsequently with a from inspiring English sources

For functionalization and recyclation of used magnetic beads, they were washed for three times with elution buffer and subsequently with a 10 mM CoCl2 solution.

For removal of the N-terminal Fmoc groups, the resin was first treated with a 20% solution of piperidine in NMP for 3 min and subsequently with a 20% solution of piperidine in NMP for 15 min.

In general, the use of deionized water often leads to an alteration of the concentration gradient between the sample and the eluent in comparison with the field situation and, subsequently, with a change in leaching behavior.

Too many families are left without their mothers, daughters, wives, sisters and friends and subsequently with a void that can never be filled.

The analyses were done with a random gene set as background, and subsequently with a muscle specific (obtained from C2C12 micro-array experiments) gene set, for confirmation.

Plates were washed with PBS and developed first with an AEC substrate kit for peroxidase (Vector, Burlingame, CA) and subsequently with a Blue alkaline phosphatase substrate kit (Vector).

The membranes were stripped and incubated with β-actin monoclonal antibody (dilution 1 5000) and subsequently with an anti-mouse antibody (dilution 1 10,000) to assess gel-loading homogeneity.

The membranes were stripped and incubated with alpha-tubulin monoclonal antibody (dilution 1 : 5000) and subsequently with an anti-mouse antibody (dilution 1 : 10000) to assess gel-loading homogeneity.

The membranes were stripped and incubated with β-actin monoclonal antibody and subsequently with an anti-mouse antibody to assess gel-loading homogeneity.

For subclass experiments, cells were incubated with isotype-specific mouse antibodies and subsequently with an Alexa Fluor 568 anti-mouse IgG.

Briefly, 15 h postirradiation, cells were fixed, and their DNA denatured, before incubating them with a primary anti-CPD antibody and, subsequently, with an appropriate secondary antibody conjugated to fluorescein isothiocyanate.