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The sample was sonicated and centrifuged at 12,000 rpm for 35 min at 48 °C for collecting the supernatant and subsequently submitting it to SDS-PAGE and Western blotting to ascertain recombinant protein expression.
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He went home (he doesn't say how) and subsequently submitted a report to Sandoz.
"The minister will consider the group's report and subsequently submit it to government," the spokesperson said.
Coatings of composite Al+50 wt% SiC were initially deposited using the high velocity oxy-fuel (HVOF) technique and subsequently submitted to shocks generated by a Nd glass laser.
In the present work, this material was chemically characterized and subsequently submitted to a dilute acid hydrolysis aiming to recover the hemicellulose sugars.
It had one PAM that was considered applicable to both indications and was therefore counted twice jIn case of no additional benefit due to missing data, the manufacturer can apply for re-assessment and subsequently submit the missing data.
By identifying these features, a 3D pharmacophore model can be built in order to screen multi-conformatorial databases with the aim to detect compounds matching the pharmacophoric hypothesis and subsequently submit them to a biological testing.
To overcome this problem, the product of the trypsin digest was treated with barium hydroxide to eliminate the phosphate groups and subsequently submitted to a 1,4-addition with 2-mercaptoethanol (Fig 4).
Using vasa::egfp transgenic zebrafish as donors, a testicular cell suspension was obtained and subsequently submitted to FACS, in order to enrich transplantable SSC (Figure 4J,K) by sorting for big cells carrying high fluorescence (Figure 4L,M).
The cultures were dark-adapted for 48 hours and subsequently submitted to either blue light (25 µmol m−2 s−1) with an emission between 380 450 nm (F 40 BB, Philips), red light (25 µmol m−2 s−1) provided by an LED lamp with a peak emission at 670 nm (QB1310CS-670-735, Quantum devInc .Inc)., or white fluorescent light (175 µmol m−2 s−1; TLD 58W/840, Philips).
Cells were washed and subsequently submitted to three-color immunofluorescence staining as previously described [ 13].
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