In the present work, this material was chemically characterized and subsequently submitted to a dilute acid hydrolysis aiming to recover the hemicellulose sugars.
To overcome this problem, the product of the trypsin digest was treated with barium hydroxide to eliminate the phosphate groups and subsequently submitted to a 1,4-addition with 2-mercaptoethanol (Fig 4).
Mass spectral raw files were then charge-deconvoluted by using Mascot Distiller software (Matrix Science, Boston, MA, USA) and subsequently submitted to a Mascot database search (Matrix Science).
The reactions were left overnight at RT before they were quenched by the addition of H2O containing 0.1% TFA to a total volume of 2 mL and subsequently submitted to HPLC (JASCO) on a C18 column.
The reaction was quenched by the addition of H2O containing 0.1% TFA to a total volume of 2 mL and subsequently submitted to HPLC (JASCO) on a C18 column.
The contrast map was initially thresholded at a voxel level t(19) = 2.860 and subsequently submitted to statistical inference with a cluster size threshold at P < 0.05 (5000 iterations), corrected for multiple comparisons.
The resulting 251-bp fluorescent PCR products (1 µl) were digested for 3 hr using 10 units of HaeIII and subsequently submitted to electrophoresis using an automated genetic analyser ABI3130 (Applied Biosystems, Norwalk, CT).
An F.D.A. official said at Thursday's hearing that Coast had subsequently submitted a plan to improve its procedures.
Coatings of composite Al+50 wt% SiC were initially deposited using the high velocity oxy-fuel (HVOF) technique and subsequently submitted to shocks generated by a Nd glass laser.
