Sentence examples for and subsequently stimulated from inspiring English sources

In stimulation experiments, the medium was changed 24 h after transfection, washed and subsequently stimulated with or without TNF-α (10 nM) for the next 24 h.

To confirm these observations, ACs were further pretreated with a selective antagonist of RAS, GGT12133 (2 or 10 μM), and subsequently stimulated for 5 or 15 minutes.

Cells were pretreated with BNO 1095 for 1 h and subsequently stimulated with 50 ng/ml LPS for 24 h.

H69 cells were subjected to serum starvation for 36 hours and subsequently stimulated with 10% FBS for 8 hours [46].

Splenocytes from naive or transplanted B6 mice were harvested and subsequently stimulated with naive wild-type bm12 splenocytes.

Macrophages were treated with 10 µg/mL AKBA for 30 min and subsequently stimulated with PL in lysine-free RPMI 1640 (Sigma) for six hours before harvesting for RNA extraction.

PAC-1 SMC were transfected with the indicated construct, made quiescent for 24 hours using 0.25% FBS-containing DMEM, and subsequently stimulated with 2×10−6 M atRA for 24 hours.

Because lipoproteins are known to bind and neutralize LPS, we preincubated Salmonella LPS and heat-killed whole Salmonella bacteria with LDL at various concentrations, and subsequently stimulated normal (LDLR+/+) macrophages for cytokine production.

(A, B ) Control (Luc shRNA) or Daple-depleted (Daple shRNA) HeLa cells were cotransfected with Gαi1-YFP, Gβ-CFP, and untagged Gγ, serum starved overnight (0.2% FBS) and subsequently stimulated with either Wnt3 and analyzed for FRET by confocal microscopy.

Cells were serum starved 2 hours before treatment and subsequently stimulated either for 4 h or 24 h.

pDCs primed with HCVcc for 2 h and subsequently stimulated with CpG-A produced IFN-α at 11.2±5.4% of the levels produced by pDCs stimulated only with CpG-A (Figure 5A).