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By constructing RTP1S deletion and chimera series and subsequently introducing single-site mutations into the protein, we found the N terminus of RTP1S is important for the endoplasmic reticulum exit of ORs and that a middle region of RTP1S is important for OR trafficking from the Golgi to the membrane.
An alternative approach was also used, namely introducing 50% diluted myosin to the flow cell, treating with donor for one minute (as above), and subsequently introducing a second bolus of 50% diluted myosin.
For example, S. coelicolor M1154 was constructed by deleting four gene clusters (actinorhodin, prodiginine, calcium-dependent antibiotic, and cryptic polyketide) and subsequently introducing point mutations in the rpoB and rpsL genes that enhance specialized metabolite production [ 125].
The model was built using a purely forward selection routine, by trying all possible combinations introducing one covariate at each time, and subsequently introducing interactions between the covariates included.
Thus, developing and subsequently introducing these reagents into routine diagnostics could represent an improvement in both strategies: control of PTB by vaccination and control of TB by rapid detection of infected animals.
These evaluations are a fundamental part of the clinical study aimed at determining the risk/benefit ratio of its use in the field of human health and subsequently introducing this extract into dietary supplement practice.
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In 1980 Roy Lancaster collected and subsequently introduced S. ruscifolia v. chinensis from Yunnan in China.
The company was in trouble during the 1990s and subsequently introduced "lean manufacturing", which focuses on efficiency and micro-management.
We began the process in this manner and subsequently introduced restrictions on certain terms to improve the search specificity.
The retained complex was eluted by 2 ml 2 mol l−1 HCl and subsequently introduced on-line into the integrated reaction chamber/gas liquid separator (RC GLS).
Three years before it bought Zagat, Google unsuccessfully sought to buy Yelp for $500 million, and subsequently introduced Google Places, a Yelp-ish service for listing local businesses and collecting consumer reviews.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com