Sentence examples for and subsequently inserted from inspiring English sources

Then, the Kan was digested with the nuclease SalI and subsequently inserted into the recombinant plasmid pMD19-Up-Down to form the plasmid pMD19-Up-Kan-Down.

Resulting PCR product was digested using XhoI and BamHI restriction enzymes, and subsequently inserted into pCDNA3-bestrophin-1 plasmid digested with XhoI/BamHI.

T6SS genes were amplified using indicated primers (see Appendix), cloned into plasmid pJet (Thermo Scientific) and subsequently inserted into pBAD24.

The resulting fragment was recombined into pDONR207 as described above and subsequently inserted into pEARLEYGATE302 using the Gateway LR clonase reaction (Invitrogen ®).

A 1977 bp D6PK promoter-PCR fragment (D6PKp) was cloned into pCR2.1 (LifeTechnologies, Carlsbad, CA) and subsequently inserted as KpnI/ XhoI fragment into pGREEN-YFP D6PK pGREEN-YFP D6PKKp:YFP:D6PK.

To make Plac- efe-Flag plasmid, the efe-Flag ORF from pJU105 was PCR amplified, digested with HindIII and KpnI and subsequently inserted into the pUC-based pGFPuv vector digested with the same enzymes.

PMMA reinforcement of pedicle screws can be carried out by first injecting the cement into the pedicle and subsequently inserting the screw.

How do tandem repeats form, and subsequently insert and delete in mt genomes, and why do they have high sequence and length heteroplasmy even within an individual?

This oligomer gains higher affinity to GPI-anchored receptors and subsequently inserts into the membrane, opening a non-selective pore [ 8].

The vector was treated with CIP (calf-intestinal alkaline phosphatase, NEB, Ipswich, MA, United States) for an additional hour and subsequently insert and vector were purified from 1% agarose gel using the QIAquick gel extraction kit followed by the Qiagen PCR purification kit.

The parasites encoding RON2mCherry in replacement of RON2 express a functional RON2 [ 22] and behave similarly as the parental strain, thereby enabling us to document dynamically that a burst of RON2 molecules traffics from the rhoptry to the tip of the extruded conoid, and subsequently inserts into the host cell plasma bilayer to initiate PV folding.