Sentence examples for and subsequently insert from inspiring English sources

How do tandem repeats form, and subsequently insert and delete in mt genomes, and why do they have high sequence and length heteroplasmy even within an individual?

The vector was treated with CIP (calf-intestinal alkaline phosphatase, NEB, Ipswich, MA, United States) for an additional hour and subsequently insert and vector were purified from 1% agarose gel using the QIAquick gel extraction kit followed by the Qiagen PCR purification kit.

Fragments of sGFP and sGFP-3′-UTR were amplified from pTKM1-sGFP-3′-UTR plasmid and cloned into pENTR/D-TOPO (Invitrogen) and subsequently inserted into the pT1OG vector (LC126301)40 (Supplementary Fig. 4a,b).

PMMA reinforcement of pedicle screws can be carried out by first injecting the cement into the pedicle and subsequently inserting the screw.

Then, the Kan was digested with the nuclease SalI and subsequently inserted into the recombinant plasmid pMD19-Up-Down to form the plasmid pMD19-Up-Kan-Down.

Resulting PCR product was digested using XhoI and BamHI restriction enzymes, and subsequently inserted into pCDNA3-bestrophin-1 plasmid digested with XhoI/BamHI.

T6SS genes were amplified using indicated primers (see Appendix), cloned into plasmid pJet (Thermo Scientific) and subsequently inserted into pBAD24.

The resulting fragment was recombined into pDONR207 as described above and subsequently inserted into pEARLEYGATE302 using the Gateway LR clonase reaction (Invitrogen ®).

This oligomer gains higher affinity to GPI-anchored receptors and subsequently inserts into the membrane, opening a non-selective pore [ 8].

A 1977 bp D6PK promoter-PCR fragment (D6PKp) was cloned into pCR2.1 (LifeTechnologies, Carlsbad, CA) and subsequently inserted as KpnI/ XhoI fragment into pGREEN-YFP D6PK pGREEN-YFP D6PKKp:YFP:D6PK.

Fragments of the heterologous promoters, except CaMV 35S long), were excised with SalI and XhoI and subsequently inserted between the SalI and XhoI sites of the three basic vectors pluc, pluc-Ω and pluc-enh, respectively.