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Then, we evaluated the open-ended questions to find recurring themes and subsequently generated a rubric to further score the survey (Additional file 1: Table S1).
We therefore obtained the complete 3′ UTR of ZEB2 by PCR amplification and subsequently generated a luciferase reporter construct as previously described [ 24].
To investigate the biological function of MAL13P1.156, we constructed a fusion construct of this protein with a C-terminal GFP and subsequently generated a P. falciparum (3D7) transgenic cell line where this fusion protein is expressed episomally.
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Thus, the rescue titer is a measure of the ability of a given vector combination to co-package and subsequently generate a provirus.
We seek the best probability model(s) for earthquake forecasting, and subsequently generate a number of conditional probability curves (hazard curves) to assess the present-day seismicity in the Kachchh region.
This is mediated by the fact that these materials absorb strongly the primary radiation beam (typically X-rays in the MeV range, although electrons, neutrons, and positron are also employed), especially high Z number atoms, and subsequently generate a cascade of secondary low-energy electrons (LEEs) highly toxic within a very short range around the NP (Pimblott and LaVerne 2007).
The DAB Peroxidase Substrate reaction mixture contains H2O2 which oxidizes DAB, and subsequently generates a dark brown reaction product when peroxidases are present.
A handheld scanner, Sense (3D Systems), was used in this study for scanning and subsequently generating an output mesh model of a limb.
Then, it automatically converts the obtained information in a form that can be reproduced immediately through a tactile printer, and subsequently generates an SVG (Scalable Vector Graphics) file, which associates textual descriptions of the directions with a recreated tactile map.
The metalloproteinase domain of the enzyme is responsible for the initial step of N-cadherin processing, which releases soluble active fragments into the extracellular space, and subsequently generates an intracellular C-terminal fragment (CTF) [ 8, 9].
The subsequently generated Ad.shERα vectors were equally effective in vitro.
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