The phrase "and subsequently further" is not correct and may be considered redundant in written English.
It can be used when discussing a sequence of events or actions that follow one another, but the word "further" is unnecessary after "subsequently."
Example: "The project was approved, and subsequently, further steps were taken to implement the plan."
Alternatives: "and then" or "and afterward".
Subdivision proceeds in stages: initially a mass of cells is set aside for an organ system (for the alimentary canal, for instance) and subsequently further subdivided into the rudiments of various parts of the organ system, such as the liver, stomach, and intestines.
Specimens were first subjected to various degrees of fatigue damage in air, then immersed in a corrosive solution for a fixed amount of time, and subsequently further fatigued in air to failure.
The resin was transferred to a column from which the viruses were eluted, and subsequently further concentrated by acid precipitation, followed by SDS-polyacrylamide gel electrophoresis and Western blot analysis.
The results with the vitro assay were confirmed, and subsequently further expanded using mature greenhouse-grown plants [27].
siRNA transfected cells were plated in a 6-well plate with 2 mL supplemented RPMI-1640 medium with 10% FBS for 15 h and subsequently further treated with or without 10 µM DX1 for 24 h.
We coded the responses given by mothers and subsequently further grouped together similar responses.
The MAb concentration provided the higher signal and was subsequently further used for sensitivity testing of S. typhimurium cells.
The PCR products were analyzed by agarose gel electrophoresis to verify that they had the expected sizes and were subsequently further verified by sequencing.
As a result of the time course experiments, HT2-3 O- β-Glc was verified as the major metabolite of HT2 and T2 metabolism which reached its maximum already 1 day after toxin treatment and was subsequently further metabolised.
As this group of methods is also rate-based, the scale of the movements are not taken into account such that an individual that was located very close to the nest and then subsequently further away would show a large change in rate due to the scale on which the displacement occurs.
Content of codes, including specific quotes, were presented and discussed at regular meetings and codes were subsequently further refined.
