Sentence examples for and subsequently concentrated from inspiring English sources
Individual DNA samples were pooled (50 ng per individual) and subsequently concentrated using a DNA concentrator Eppendorf 5301.
The eluted and neutralized samples were combined, diluted to 20ml with MilliQ water and subsequently concentrated to the original starting volume using centrifugal concentration tubes [Vivaspin 20, Sartorius AG, Goettingen, Germany: Code no VS2021].
The protein was cleared of imidazole using dialysis with phosphate buffered saline and subsequently concentrated using a 9 kDa cut-off protein concentrator according to the manufacturer's instructions (Thermo Fisher Scientific, Rockford, IL, USA).
However, the nematodes in the cited study were obtained by extraction from large quantities of roots, and subsequently concentrated to rather high densities before inoculation.
The solution was stirred for 5 6 h at room temperature and subsequently concentrated in vacuum.
Detergent solubilized whole cell extracts were prepared, clarified by microcentrifugation, and subsequently concentrated by acetone precipitation.
Refolding was attempted using the detergent dilution method that has been successful for refolding other helical membrane proteins [20,21]; the SDS concentration was diluted to below its cmc with 20 mM Tris buffer, pH 7.5 containing 0.1% DDM and subsequently concentrating the sample using a concentrator with a 100 kDa cut-off membrane (Millipore) to ∼ 100 μl.
The paper gives an overview of the CMS tracker system and subsequently concentrates on the results from the sensor tests.
Thus it appears to localize to brain microvasculature and subsequently concentrates in brain parenchyma [52].
The complex eluted in a single peak with apparently stoichiometric amounts of Bub1289 359 and Bub3 and was subsequently concentrated to ∼10 mg/ml for crystallization or to 2 4 mg/ml for ITC experiments.
The fractions containing proteins (MBP-ΔN597 or MBP-ΔNCP69) were confirmed by SDS PAGE, and were subsequently concentrated using 10 kDa MWCO Vivaspin Sample Concentrators (Sartorius Stedim Biotech).
