Sentence examples for and subsequent with from inspiring English sources

Refixation was carried out with cold (-20°C) acetone for 5 minutes followed by 3 times washing in PBS and subsequent with PBS containing 1% BSA.

But when McCourt bought the Dodgers in 2004, he fired Evans and replaced him with DePodesta, and subsequent dealings with Boras left the Dodgers bruised anew.

The absorbance of chromophore form during the diazotization of nitrite with sulphanilamide and subsequent coupling with naphthyl-ethylenediamine was read at 546 nm.

The absorbance of the chromophore formed during diazotization of the nitrite with sulphanilamide and subsequent coupling with naphthylethylenediaminedihydrochloride was read at 546 nm.

The absorbance of the chromophore that formed during diazotization of the nitrite with sulfanilamide and subsequent coupling with naphthylethylenediamine dihydrochloride was immediately read at 550 nm.

Activation of the carboxylic groups with N,N′-carbonyldiimidazol and subsequent coupling with amines is discussed.

And now comes news of his visits to Libya (and subsequent briefings with Vice President Cheney), with Perle acting once again as, reportedly, an unregistered agent of influence.

Immunoprecipitation of control or sFLT1-treated cell lysates with anti-KDR and subsequent detection with anti-FLT1 confirmed this.

Slides were briefly washed with water prior to drying and subsequent scanning with a Genepix 4000A scanner (MDS, Sunnyvale California).

Washing the column with water removed the inorganic salts and subsequent elution with methanol allowed to recover saponins.

One thing very few would argue with is that Evans handled his departure and subsequent criticism with a rare level of dignity.